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paper n a tzm bl  (ATCC)
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Paper N A Tzm Bl, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paper n a recombinant dna pcmv3 human coch myc sino biological  (Sino Biological)
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Sino Biological paper n a recombinant dna pcmv3 human coch myc sino biological
Paper N A Recombinant Dna Pcmv3 Human Coch Myc Sino Biological, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paper n a cdna rat lamp1  (Addgene inc)
93
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Figure 3. Autophagosomes Accumulate in Periaxonal Soma of DKD Neurons (A) Images of DIV14 <t>mCh-Lamp1/SC</t> (top) and mCh-Lamp1/DKD (bottom) neurons immunostained with Ankyrin-G (arrows) to mark the initial segment of the axon. mCh-Lamp1 puncta are clustered in the periaxonal soma of the DKD neuron. (B) Quantitation of Lamp1 asymmetry in SC and DKD neurons shows an increase in axon-proximal mCh-Lamp1 in DKD neurons. (C) Images of SC and DKD neuronal cell soma (DIV14) labeled with Lysotracker reveals increased axon-proximally located lysosomes in DKD neuron. (D) Quantitation of axon-proximal versus axon-distal Lysotracker puncta in EGFP SC- and EGFP DKD-expressing neurons is shown. (E) Mature autolysosomes, identified as mRFP+/EGFP puncta, were asymmetrically distributed in the soma of tf-LC3 and DKD neurons (DIV14). The addition of 100 nM bafilomycin to DKD neurons (lower panel) increased the number of EGFP+ puncta in DKD neurons. (F) Quantitation of fluorescent puncta from tf-LC3 and DKD neurons treated with DMSO or DMSO and 100 nM bafilomycin A for 3 hr shows a marked increase in mRFP+/EGFP+ puncta after bafilomycin administration. (G) EM micrograph of the cell soma of a DIV14 VAMP2-HRP/DKD neuron containing DAB-labeled vesicular structures as cargo in an autophagic vacuole-like structure. N marks the cell nucleus. DAB-labeled structures were not observed in the soma of any VAMP2-HRP/SC neurons. Scale bars, 10 mm (A, C, and G) and 500 nm (G). Graphs represent mean ± SEM. See Table S1 for means ± SEM, scoring, and n and p values. See also Figure S3 with Movies S1 and S2 and Figure S4.
Paper N A Cdna Rat Lamp1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paper n a recombinant dna tet plko 1 puro shluc  (Addgene inc)
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Figure 3. Autophagosomes Accumulate in Periaxonal Soma of DKD Neurons (A) Images of DIV14 <t>mCh-Lamp1/SC</t> (top) and mCh-Lamp1/DKD (bottom) neurons immunostained with Ankyrin-G (arrows) to mark the initial segment of the axon. mCh-Lamp1 puncta are clustered in the periaxonal soma of the DKD neuron. (B) Quantitation of Lamp1 asymmetry in SC and DKD neurons shows an increase in axon-proximal mCh-Lamp1 in DKD neurons. (C) Images of SC and DKD neuronal cell soma (DIV14) labeled with Lysotracker reveals increased axon-proximally located lysosomes in DKD neuron. (D) Quantitation of axon-proximal versus axon-distal Lysotracker puncta in EGFP SC- and EGFP DKD-expressing neurons is shown. (E) Mature autolysosomes, identified as mRFP+/EGFP puncta, were asymmetrically distributed in the soma of tf-LC3 and DKD neurons (DIV14). The addition of 100 nM bafilomycin to DKD neurons (lower panel) increased the number of EGFP+ puncta in DKD neurons. (F) Quantitation of fluorescent puncta from tf-LC3 and DKD neurons treated with DMSO or DMSO and 100 nM bafilomycin A for 3 hr shows a marked increase in mRFP+/EGFP+ puncta after bafilomycin administration. (G) EM micrograph of the cell soma of a DIV14 VAMP2-HRP/DKD neuron containing DAB-labeled vesicular structures as cargo in an autophagic vacuole-like structure. N marks the cell nucleus. DAB-labeled structures were not observed in the soma of any VAMP2-HRP/SC neurons. Scale bars, 10 mm (A, C, and G) and 500 nm (G). Graphs represent mean ± SEM. See Table S1 for means ± SEM, scoring, and n and p values. See also Figure S3 with Movies S1 and S2 and Figure S4.
Paper N A Recombinant Dna Tet Plko 1 Puro Shluc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paper n a software  (Oxford Instruments)
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Oxford Instruments paper n a software
Figure 3. Autophagosomes Accumulate in Periaxonal Soma of DKD Neurons (A) Images of DIV14 <t>mCh-Lamp1/SC</t> (top) and mCh-Lamp1/DKD (bottom) neurons immunostained with Ankyrin-G (arrows) to mark the initial segment of the axon. mCh-Lamp1 puncta are clustered in the periaxonal soma of the DKD neuron. (B) Quantitation of Lamp1 asymmetry in SC and DKD neurons shows an increase in axon-proximal mCh-Lamp1 in DKD neurons. (C) Images of SC and DKD neuronal cell soma (DIV14) labeled with Lysotracker reveals increased axon-proximally located lysosomes in DKD neuron. (D) Quantitation of axon-proximal versus axon-distal Lysotracker puncta in EGFP SC- and EGFP DKD-expressing neurons is shown. (E) Mature autolysosomes, identified as mRFP+/EGFP puncta, were asymmetrically distributed in the soma of tf-LC3 and DKD neurons (DIV14). The addition of 100 nM bafilomycin to DKD neurons (lower panel) increased the number of EGFP+ puncta in DKD neurons. (F) Quantitation of fluorescent puncta from tf-LC3 and DKD neurons treated with DMSO or DMSO and 100 nM bafilomycin A for 3 hr shows a marked increase in mRFP+/EGFP+ puncta after bafilomycin administration. (G) EM micrograph of the cell soma of a DIV14 VAMP2-HRP/DKD neuron containing DAB-labeled vesicular structures as cargo in an autophagic vacuole-like structure. N marks the cell nucleus. DAB-labeled structures were not observed in the soma of any VAMP2-HRP/SC neurons. Scale bars, 10 mm (A, C, and G) and 500 nm (G). Graphs represent mean ± SEM. See Table S1 for means ± SEM, scoring, and n and p values. See also Figure S3 with Movies S1 and S2 and Figure S4.
Paper N A Software, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paper n a hek293t atcc  (ATCC)
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ATCC paper n a hek293t atcc
Figure 3. Autophagosomes Accumulate in Periaxonal Soma of DKD Neurons (A) Images of DIV14 <t>mCh-Lamp1/SC</t> (top) and mCh-Lamp1/DKD (bottom) neurons immunostained with Ankyrin-G (arrows) to mark the initial segment of the axon. mCh-Lamp1 puncta are clustered in the periaxonal soma of the DKD neuron. (B) Quantitation of Lamp1 asymmetry in SC and DKD neurons shows an increase in axon-proximal mCh-Lamp1 in DKD neurons. (C) Images of SC and DKD neuronal cell soma (DIV14) labeled with Lysotracker reveals increased axon-proximally located lysosomes in DKD neuron. (D) Quantitation of axon-proximal versus axon-distal Lysotracker puncta in EGFP SC- and EGFP DKD-expressing neurons is shown. (E) Mature autolysosomes, identified as mRFP+/EGFP puncta, were asymmetrically distributed in the soma of tf-LC3 and DKD neurons (DIV14). The addition of 100 nM bafilomycin to DKD neurons (lower panel) increased the number of EGFP+ puncta in DKD neurons. (F) Quantitation of fluorescent puncta from tf-LC3 and DKD neurons treated with DMSO or DMSO and 100 nM bafilomycin A for 3 hr shows a marked increase in mRFP+/EGFP+ puncta after bafilomycin administration. (G) EM micrograph of the cell soma of a DIV14 VAMP2-HRP/DKD neuron containing DAB-labeled vesicular structures as cargo in an autophagic vacuole-like structure. N marks the cell nucleus. DAB-labeled structures were not observed in the soma of any VAMP2-HRP/SC neurons. Scale bars, 10 mm (A, C, and G) and 500 nm (G). Graphs represent mean ± SEM. See Table S1 for means ± SEM, scoring, and n and p values. See also Figure S3 with Movies S1 and S2 and Figure S4.
Paper N A Hek293t Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paper n a plasmid  (Addgene inc)
91
Addgene inc paper n a plasmid
Figure 3. Autophagosomes Accumulate in Periaxonal Soma of DKD Neurons (A) Images of DIV14 <t>mCh-Lamp1/SC</t> (top) and mCh-Lamp1/DKD (bottom) neurons immunostained with Ankyrin-G (arrows) to mark the initial segment of the axon. mCh-Lamp1 puncta are clustered in the periaxonal soma of the DKD neuron. (B) Quantitation of Lamp1 asymmetry in SC and DKD neurons shows an increase in axon-proximal mCh-Lamp1 in DKD neurons. (C) Images of SC and DKD neuronal cell soma (DIV14) labeled with Lysotracker reveals increased axon-proximally located lysosomes in DKD neuron. (D) Quantitation of axon-proximal versus axon-distal Lysotracker puncta in EGFP SC- and EGFP DKD-expressing neurons is shown. (E) Mature autolysosomes, identified as mRFP+/EGFP puncta, were asymmetrically distributed in the soma of tf-LC3 and DKD neurons (DIV14). The addition of 100 nM bafilomycin to DKD neurons (lower panel) increased the number of EGFP+ puncta in DKD neurons. (F) Quantitation of fluorescent puncta from tf-LC3 and DKD neurons treated with DMSO or DMSO and 100 nM bafilomycin A for 3 hr shows a marked increase in mRFP+/EGFP+ puncta after bafilomycin administration. (G) EM micrograph of the cell soma of a DIV14 VAMP2-HRP/DKD neuron containing DAB-labeled vesicular structures as cargo in an autophagic vacuole-like structure. N marks the cell nucleus. DAB-labeled structures were not observed in the soma of any VAMP2-HRP/SC neurons. Scale bars, 10 mm (A, C, and G) and 500 nm (G). Graphs represent mean ± SEM. See Table S1 for means ± SEM, scoring, and n and p values. See also Figure S3 with Movies S1 and S2 and Figure S4.
Paper N A Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paper n a mouse gli1 kr mutations  (OriGene)
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OriGene paper n a mouse gli1 kr mutations
Figure 3. Autophagosomes Accumulate in Periaxonal Soma of DKD Neurons (A) Images of DIV14 <t>mCh-Lamp1/SC</t> (top) and mCh-Lamp1/DKD (bottom) neurons immunostained with Ankyrin-G (arrows) to mark the initial segment of the axon. mCh-Lamp1 puncta are clustered in the periaxonal soma of the DKD neuron. (B) Quantitation of Lamp1 asymmetry in SC and DKD neurons shows an increase in axon-proximal mCh-Lamp1 in DKD neurons. (C) Images of SC and DKD neuronal cell soma (DIV14) labeled with Lysotracker reveals increased axon-proximally located lysosomes in DKD neuron. (D) Quantitation of axon-proximal versus axon-distal Lysotracker puncta in EGFP SC- and EGFP DKD-expressing neurons is shown. (E) Mature autolysosomes, identified as mRFP+/EGFP puncta, were asymmetrically distributed in the soma of tf-LC3 and DKD neurons (DIV14). The addition of 100 nM bafilomycin to DKD neurons (lower panel) increased the number of EGFP+ puncta in DKD neurons. (F) Quantitation of fluorescent puncta from tf-LC3 and DKD neurons treated with DMSO or DMSO and 100 nM bafilomycin A for 3 hr shows a marked increase in mRFP+/EGFP+ puncta after bafilomycin administration. (G) EM micrograph of the cell soma of a DIV14 VAMP2-HRP/DKD neuron containing DAB-labeled vesicular structures as cargo in an autophagic vacuole-like structure. N marks the cell nucleus. DAB-labeled structures were not observed in the soma of any VAMP2-HRP/SC neurons. Scale bars, 10 mm (A, C, and G) and 500 nm (G). Graphs represent mean ± SEM. See Table S1 for means ± SEM, scoring, and n and p values. See also Figure S3 with Movies S1 and S2 and Figure S4.
Paper N A Mouse Gli1 Kr Mutations, supplied by OriGene, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phenyl sepharose 6 fast flow  (GE Healthcare)
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Figure 3. Autophagosomes Accumulate in Periaxonal Soma of DKD Neurons (A) Images of DIV14 <t>mCh-Lamp1/SC</t> (top) and mCh-Lamp1/DKD (bottom) neurons immunostained with Ankyrin-G (arrows) to mark the initial segment of the axon. mCh-Lamp1 puncta are clustered in the periaxonal soma of the DKD neuron. (B) Quantitation of Lamp1 asymmetry in SC and DKD neurons shows an increase in axon-proximal mCh-Lamp1 in DKD neurons. (C) Images of SC and DKD neuronal cell soma (DIV14) labeled with Lysotracker reveals increased axon-proximally located lysosomes in DKD neuron. (D) Quantitation of axon-proximal versus axon-distal Lysotracker puncta in EGFP SC- and EGFP DKD-expressing neurons is shown. (E) Mature autolysosomes, identified as mRFP+/EGFP puncta, were asymmetrically distributed in the soma of tf-LC3 and DKD neurons (DIV14). The addition of 100 nM bafilomycin to DKD neurons (lower panel) increased the number of EGFP+ puncta in DKD neurons. (F) Quantitation of fluorescent puncta from tf-LC3 and DKD neurons treated with DMSO or DMSO and 100 nM bafilomycin A for 3 hr shows a marked increase in mRFP+/EGFP+ puncta after bafilomycin administration. (G) EM micrograph of the cell soma of a DIV14 VAMP2-HRP/DKD neuron containing DAB-labeled vesicular structures as cargo in an autophagic vacuole-like structure. N marks the cell nucleus. DAB-labeled structures were not observed in the soma of any VAMP2-HRP/SC neurons. Scale bars, 10 mm (A, C, and G) and 500 nm (G). Graphs represent mean ± SEM. See Table S1 for means ± SEM, scoring, and n and p values. See also Figure S3 with Movies S1 and S2 and Figure S4.
Phenyl Sepharose 6 Fast Flow, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paper n a pcmv vsv g addgene  (Addgene inc)
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Figure 3. Autophagosomes Accumulate in Periaxonal Soma of DKD Neurons (A) Images of DIV14 <t>mCh-Lamp1/SC</t> (top) and mCh-Lamp1/DKD (bottom) neurons immunostained with Ankyrin-G (arrows) to mark the initial segment of the axon. mCh-Lamp1 puncta are clustered in the periaxonal soma of the DKD neuron. (B) Quantitation of Lamp1 asymmetry in SC and DKD neurons shows an increase in axon-proximal mCh-Lamp1 in DKD neurons. (C) Images of SC and DKD neuronal cell soma (DIV14) labeled with Lysotracker reveals increased axon-proximally located lysosomes in DKD neuron. (D) Quantitation of axon-proximal versus axon-distal Lysotracker puncta in EGFP SC- and EGFP DKD-expressing neurons is shown. (E) Mature autolysosomes, identified as mRFP+/EGFP puncta, were asymmetrically distributed in the soma of tf-LC3 and DKD neurons (DIV14). The addition of 100 nM bafilomycin to DKD neurons (lower panel) increased the number of EGFP+ puncta in DKD neurons. (F) Quantitation of fluorescent puncta from tf-LC3 and DKD neurons treated with DMSO or DMSO and 100 nM bafilomycin A for 3 hr shows a marked increase in mRFP+/EGFP+ puncta after bafilomycin administration. (G) EM micrograph of the cell soma of a DIV14 VAMP2-HRP/DKD neuron containing DAB-labeled vesicular structures as cargo in an autophagic vacuole-like structure. N marks the cell nucleus. DAB-labeled structures were not observed in the soma of any VAMP2-HRP/SC neurons. Scale bars, 10 mm (A, C, and G) and 500 nm (G). Graphs represent mean ± SEM. See Table S1 for means ± SEM, scoring, and n and p values. See also Figure S3 with Movies S1 and S2 and Figure S4.
Paper N A Pcmv Vsv G Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paper n a trem2 standard peptide sinobiological  (Sino Biological)
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Figure 3. Autophagosomes Accumulate in Periaxonal Soma of DKD Neurons (A) Images of DIV14 <t>mCh-Lamp1/SC</t> (top) and mCh-Lamp1/DKD (bottom) neurons immunostained with Ankyrin-G (arrows) to mark the initial segment of the axon. mCh-Lamp1 puncta are clustered in the periaxonal soma of the DKD neuron. (B) Quantitation of Lamp1 asymmetry in SC and DKD neurons shows an increase in axon-proximal mCh-Lamp1 in DKD neurons. (C) Images of SC and DKD neuronal cell soma (DIV14) labeled with Lysotracker reveals increased axon-proximally located lysosomes in DKD neuron. (D) Quantitation of axon-proximal versus axon-distal Lysotracker puncta in EGFP SC- and EGFP DKD-expressing neurons is shown. (E) Mature autolysosomes, identified as mRFP+/EGFP puncta, were asymmetrically distributed in the soma of tf-LC3 and DKD neurons (DIV14). The addition of 100 nM bafilomycin to DKD neurons (lower panel) increased the number of EGFP+ puncta in DKD neurons. (F) Quantitation of fluorescent puncta from tf-LC3 and DKD neurons treated with DMSO or DMSO and 100 nM bafilomycin A for 3 hr shows a marked increase in mRFP+/EGFP+ puncta after bafilomycin administration. (G) EM micrograph of the cell soma of a DIV14 VAMP2-HRP/DKD neuron containing DAB-labeled vesicular structures as cargo in an autophagic vacuole-like structure. N marks the cell nucleus. DAB-labeled structures were not observed in the soma of any VAMP2-HRP/SC neurons. Scale bars, 10 mm (A, C, and G) and 500 nm (G). Graphs represent mean ± SEM. See Table S1 for means ± SEM, scoring, and n and p values. See also Figure S3 with Movies S1 and S2 and Figure S4.
Paper N A Trem2 Standard Peptide Sinobiological, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human recombinant insulin  (Millipore)
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Millipore human recombinant insulin
Figure 3. Autophagosomes Accumulate in Periaxonal Soma of DKD Neurons (A) Images of DIV14 <t>mCh-Lamp1/SC</t> (top) and mCh-Lamp1/DKD (bottom) neurons immunostained with Ankyrin-G (arrows) to mark the initial segment of the axon. mCh-Lamp1 puncta are clustered in the periaxonal soma of the DKD neuron. (B) Quantitation of Lamp1 asymmetry in SC and DKD neurons shows an increase in axon-proximal mCh-Lamp1 in DKD neurons. (C) Images of SC and DKD neuronal cell soma (DIV14) labeled with Lysotracker reveals increased axon-proximally located lysosomes in DKD neuron. (D) Quantitation of axon-proximal versus axon-distal Lysotracker puncta in EGFP SC- and EGFP DKD-expressing neurons is shown. (E) Mature autolysosomes, identified as mRFP+/EGFP puncta, were asymmetrically distributed in the soma of tf-LC3 and DKD neurons (DIV14). The addition of 100 nM bafilomycin to DKD neurons (lower panel) increased the number of EGFP+ puncta in DKD neurons. (F) Quantitation of fluorescent puncta from tf-LC3 and DKD neurons treated with DMSO or DMSO and 100 nM bafilomycin A for 3 hr shows a marked increase in mRFP+/EGFP+ puncta after bafilomycin administration. (G) EM micrograph of the cell soma of a DIV14 VAMP2-HRP/DKD neuron containing DAB-labeled vesicular structures as cargo in an autophagic vacuole-like structure. N marks the cell nucleus. DAB-labeled structures were not observed in the soma of any VAMP2-HRP/SC neurons. Scale bars, 10 mm (A, C, and G) and 500 nm (G). Graphs represent mean ± SEM. See Table S1 for means ± SEM, scoring, and n and p values. See also Figure S3 with Movies S1 and S2 and Figure S4.
Human Recombinant Insulin, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 3. Autophagosomes Accumulate in Periaxonal Soma of DKD Neurons (A) Images of DIV14 mCh-Lamp1/SC (top) and mCh-Lamp1/DKD (bottom) neurons immunostained with Ankyrin-G (arrows) to mark the initial segment of the axon. mCh-Lamp1 puncta are clustered in the periaxonal soma of the DKD neuron. (B) Quantitation of Lamp1 asymmetry in SC and DKD neurons shows an increase in axon-proximal mCh-Lamp1 in DKD neurons. (C) Images of SC and DKD neuronal cell soma (DIV14) labeled with Lysotracker reveals increased axon-proximally located lysosomes in DKD neuron. (D) Quantitation of axon-proximal versus axon-distal Lysotracker puncta in EGFP SC- and EGFP DKD-expressing neurons is shown. (E) Mature autolysosomes, identified as mRFP+/EGFP puncta, were asymmetrically distributed in the soma of tf-LC3 and DKD neurons (DIV14). The addition of 100 nM bafilomycin to DKD neurons (lower panel) increased the number of EGFP+ puncta in DKD neurons. (F) Quantitation of fluorescent puncta from tf-LC3 and DKD neurons treated with DMSO or DMSO and 100 nM bafilomycin A for 3 hr shows a marked increase in mRFP+/EGFP+ puncta after bafilomycin administration. (G) EM micrograph of the cell soma of a DIV14 VAMP2-HRP/DKD neuron containing DAB-labeled vesicular structures as cargo in an autophagic vacuole-like structure. N marks the cell nucleus. DAB-labeled structures were not observed in the soma of any VAMP2-HRP/SC neurons. Scale bars, 10 mm (A, C, and G) and 500 nm (G). Graphs represent mean ± SEM. See Table S1 for means ± SEM, scoring, and n and p values. See also Figure S3 with Movies S1 and S2 and Figure S4.

Journal: Neuron

Article Title: Bassoon Controls Presynaptic Autophagy through Atg5.

doi: 10.1016/j.neuron.2017.01.026

Figure Lengend Snippet: Figure 3. Autophagosomes Accumulate in Periaxonal Soma of DKD Neurons (A) Images of DIV14 mCh-Lamp1/SC (top) and mCh-Lamp1/DKD (bottom) neurons immunostained with Ankyrin-G (arrows) to mark the initial segment of the axon. mCh-Lamp1 puncta are clustered in the periaxonal soma of the DKD neuron. (B) Quantitation of Lamp1 asymmetry in SC and DKD neurons shows an increase in axon-proximal mCh-Lamp1 in DKD neurons. (C) Images of SC and DKD neuronal cell soma (DIV14) labeled with Lysotracker reveals increased axon-proximally located lysosomes in DKD neuron. (D) Quantitation of axon-proximal versus axon-distal Lysotracker puncta in EGFP SC- and EGFP DKD-expressing neurons is shown. (E) Mature autolysosomes, identified as mRFP+/EGFP puncta, were asymmetrically distributed in the soma of tf-LC3 and DKD neurons (DIV14). The addition of 100 nM bafilomycin to DKD neurons (lower panel) increased the number of EGFP+ puncta in DKD neurons. (F) Quantitation of fluorescent puncta from tf-LC3 and DKD neurons treated with DMSO or DMSO and 100 nM bafilomycin A for 3 hr shows a marked increase in mRFP+/EGFP+ puncta after bafilomycin administration. (G) EM micrograph of the cell soma of a DIV14 VAMP2-HRP/DKD neuron containing DAB-labeled vesicular structures as cargo in an autophagic vacuole-like structure. N marks the cell nucleus. DAB-labeled structures were not observed in the soma of any VAMP2-HRP/SC neurons. Scale bars, 10 mm (A, C, and G) and 500 nm (G). Graphs represent mean ± SEM. See Table S1 for means ± SEM, scoring, and n and p values. See also Figure S3 with Movies S1 and S2 and Figure S4.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Human: HeLa, Passage < 30 ATCC CRL-7923 Experimental Models: Organisms/Strains Wildtype rats: Sprague Dawley Charles River, Harlan N/A Wildtype mice: C57Bl6/N Research Institutes for experimental Medicine (FEM) Charité Berlin N/A Bassoon knockout mice Leibniz Institute for Neurobiology Magdeburg: laboratory of Eckart D. Gundelfinger (Davydova et al., 2014) Omnibank ES cell line OST486029 by Lexicon Pharmaceuticals (The Woodlands, TX, USA) Lentivirus Garner and Reimer labs, Stanford University; Virus Core Facility, Charité Berlin N/A Helper-dependent adenovirus (HdAd) Philip Ng, Baylor College of Medicine; (Palmer & Ng 2011) N/A Recombinant DNA FU-SV2-eGFP DKD/SC-W Waites et al., 2013 N/A FU-Vamp2-HRP DKD/SC-W Waites et al., 2013 N/A FU-SV2-eGFP BsnKD-W Waites et al., 2013 N/A FU-eGFP DKD/SC-Wm Waites et al., 2013 N/A FU-mCherry-HA-Ubiquitin K0-W Waites et al., 2013 N/A FU-eGFP-Synapsin1a-W Leal-Ortiz et al., 2008 N/A FU-Synapsin1a Siah1KD-Wm This paper N/A pBsnCC2-eGFP (aa2087-2562, CAA76287.1) Maas et al., 2012 N/A pBsnZF-eGFP (aa1-609, CAA76287.1) Maas et al., 2012 N/A pBsnCD-eGFP(aa1691-3263, CAA76287.1) Maas et al., 2012 N/A pBsnFL-eGFP (aa95-3938, CAA76287.1) Maas et al., 2012 N/A FU-tf-LC3 DKD/SC-Wm This paper ptf-LC3 from Addgene #21073 FU-mRFP-LC3 DKD/SC-Wm This paper mRFP-LC3 from Addgene #21075 FU-mCh-Atg5 DKD/SC-Wm This paper mCh-Atg5 from Addgene #13095 FU-mCh-Lamp1 DKD/SC-Wm This paper pSPAX2 from Addgene #12260 FU-tf-LC3 –Wm This paper ptf-LC3 from Addgene #21073 FU-mRFP-LC3 –Wm This paper mRFP-LC3 from Addgene #21075 FU-mCh-Atg5 –Wm This paper mCh-Atg5 from Addgene #13095 FU-eGFP shAtg5-Wm This paper N/A cDNA rat Lamp1 (NM_012857.2) This paper N/A Lamp1-mCherry This paper pmCherry from Clontech Cat# 632524 FU CFP-SV2 DKD/SC-Wm Noam E. Ziv, Technion, Institute of Technology Haifa Israel N/A FU-CC2v1-eGFP-Synapsin1a-W This paper N/A FU-CC2v1*-eGFP-Synapsin1a-W This paper N/A mRFP-Bassoon* FL Waites et al., 2013 N/A FU-mCherry-Wm This paper N/A pEGFP-N Clontech Cat# 632469 pCMV-VSVg Generous gift of Dr. Theo Palmer; Stanford University N/A Sequence-Based Reagents Atg5 shRNA Obtained from the Stanford Protein and Nucleic Acid facility according to Lee and Gao, 2009 N/A fw 50-gatccccatctgagctatccagacaattcaagag attgtctggatagctcagattttttggaaa-30 Obtained from the Stanford Protein and Nucleic Acid facility according to Lee and Gao, 2009 N/A (Continued on next page) Neuron 93, 897–913.e1–e7, February 22, 2017 e2

Techniques: Quantitation Assay, Labeling, Expressing